Item Details

Title: Variability of Sweetpotato Feathery Mottle Virus in Africa

Date Published: 2001
Author/s: R. F. Karyeija, J, F, Kreuze, R. W. Gibson and J. P. T. Valkonen
Data publication:
Funding Agency :
Copyright/patents/trade marks:
Journal Publisher: African Crop Science Journal
Affiliation: Kawanda Agricultural Research Institute, P. O. Box 7065, Kampala, Uganda
1Intitute of Biotechnology, University of Helsinki, Finland
2Department of Plant Biology, Genetic Centre, Swedish University of Agricultural Sciences
(SLU), Box 7080, S-750 07, Uppsala, Sweden
3Natural Resources Institute, University of Greenwich, Central Avenue, Chatham Maritime
Keywords: Clustering, coat protein, East African SPFMV isolates, resistant sweetpotato
cultivars, sweetpotato chlorotic stunt Crinivirus, West African SPFMV isolates Mab + isolates


One sweetpotato breeding line resistant to sweetpotato feathery mottle virus (SPFMV) was
infected by graft-inoculation with SPFMV and developed severe symptoms of sweetpotato virus
disease, following co-inoculation with sweetpotato chlorotic stunt virus. Coat protein (CP) gene
sequences from eight East African isolates of SPFMV and two West Africa isolates from Nigeria
and Niger were determined. Phylogenetic analysis of sequences of these isolates and those of
non-East African origin previously reported, showed that the East African SPFMV isolates form
a separate cluster. The monoclonal antibody MAb 7H8 divided Uganda SPFMV isolates into
serogroups, which differed in prevalence in different districts of Uganda and in two common sweetpotato cultivars.