Item Details

Title: Inhibition of Agrobacterium-Induced Cell Death by Antiapoptotic Gene Expression Leads to Very High Transformation Efficiency of Banana

Date Published: 2007
Author/s: Harjeet Khanna, Jean-Yves Paul, Robert M. Harding, Martin B. Dickman, and James L. Dale
Data publication:
Funding Agency : Bill and Melinda Gates Foundation and Australian Research Council
Copyright/patents/trade marks: The American Phytopathological Society
Journal Publisher: Molecular Plant-Microbe Interactions
Affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, 2 George St., Brisbane Queensland, 4001, Australia; 2Texas A&M University, Institute for Plant Genomics and Biotechnology, Department of Plant Pathology and Microbiology, College Station 77843, U.S.A.
Keywords: necrosis


The death of plant cells in culture following exposure to Agrobacterium tumefaciens remains a major obstacle in developing Agrobacterium-mediated transformation into a highly efficient genotype-independent technology. Here, we present evidence that A. tumefaciens exposure induces cell death in banana cell suspensions. More than 90% of embryogenic banana cells died after exposure to A. tumefaciens and cell death was accompanied by a subset of features associated with apoptosis in mammalian cells, including DNA laddering, fragmentation, and formation of apoptoticlike bodies. Importantly, these cellular responses were inhibited in cells expressing the animal antiapoptosis genes Bcl-xL, Bcl-2 3' untranslated region, and CED-9. Inhibition of cell death resulted in up to 90% of cell clumps transformed with Bcl-xL, a 100-fold enhancement over vector controls, approaching the transformation and regeneration
of every “transformable” cell. Similar results using sugarcane, a crop plant known for recalcitrance to Agrobacterium transformation, suggest that antiapoptosis genes may inhibit these phenomena and increase the transformation frequency of many recalcitrant plant species, including the
major monocot cereal crop plants. Evidence of inhibition of plant cell death by cross-kingdom antiapoptotic genes also contributes to the growing evidence that genes for control of programmed cell death are conserved across wide evolutionary distances, even though these mechanisms are
not well understood in plants.