Item Details

Title: Genome-Wide Sequencing Reveals Two Major Sub-Lineages
in the Genetically Monomorphic Pathogen Xanthomonas
Campestris Pathovar Musacearum

Date Published: 2012
Author/s: Arthur Wasukira, Johnbosco Tayebwa, Richard Thwaites, Konrad Paszkiewicz,
Valente Aritua, Jerome Kubiriba, Julian Smith, Murray Grant and
David J. Studholme
Data publication:
Funding Agency : MDPI, Basel, Switzerland
Copyright/patents/trade marks: MDPI
Journal Publisher: MDPI, Basel, Switzerland
Affiliation: University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK, National Crops Resources Research Institute (NaCRRI), Kampala 7084, Uganda, The Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, UK, International Centre for Tropical Agriculture (CIAT), Kawanda Agricultural
Research Institute, Kampala 6247, Uganda
Keywords: banana Xanthomonas wilt; whole-genome sequencing; SNP; molecular
markers; Ensete ventricosum; Xanthomonas vasicola


The bacterium Xanthomonas campestris pathovar musacearum (Xcm) is the
causal agent of banana Xanthomonas wilt (BXW). This disease has devastated economies
based on banana and plantain crops (Musa species) in East Africa. Here we use genome-wide
sequencing to discover a set of single-nucleotide polymorphisms (SNPs) among East
African isolates of Xcm. These SNPs have potential as molecular markers for phylogeographic
studies of the epidemiology and spread of the pathogen. Our analysis reveals two major
sub-lineages of the pathogen, suggesting that the current outbreaks of BXW on Musa
species in the region may have more than one introductory event, perhaps from Ethiopia.
Also, based on comparisons of genome-wide sequence data from multiple isolates of Xcm
and multiple strains of X. vasicola pathovar vasculorum, we identify genes specific to Xcm
that could be used to specifically detect Xcm by PCR-based methods.