Item Details

Title: Influence of rooting media and indole-3-butyric acid (IBA) concentration on rooting and shoot formation of Warburgia ugandensis stem cuttings

Date Published: 2011
Author/s: Florence Akwatulira, Samson Gwali, John Bosco Lamoris Okullo, Paul Ssegawa, Susan
Balaba Tumwebaze, John Richard Mbwambo and Alice Muchugi6
Data publication:
Funding Agency : Lake Victoria Research Initiative (VicRes)
Copyright/patents/trade marks:
Journal Publisher: African Journal of Plant Science
Affiliation: 1Department of Forest Biology and Ecosystems Management, Faculty of Forestry and Nature Conservation, Makerere
University, P. O. Box 7062, Kampala, Uganda.
2National Forestry Resources Research Institute (NaFORRI), P. O. Box 1752, Kampala, Uganda.
3Department of Botany, Faculty of Science, Makerere University, P. O. Box 7062, Kampala, Uganda.
4Department of Forest Management, Faculty of Forestry and Nature Conservation, Makerere University, P. O. Box 7062,
Kampala, Uganda.
5Lushoto Forestry Research Station, Tanzania Forestry Research Institute (TAFORI), P. O. Box 95, Lushoto, Tanzania.
6Department of Biochemistry and Biotechnology, Faculty of Science, Kenyatta University, P. O. Box 43844-00100,
Nairobi, Kenya.
Keywords: Warburgia ugandensis, indolebutryic acid (IBA), vegetative propagation, callusing, rooting, milled pine bark.

Abstract:

This study investigated the influence of different rooting media and indolebutryic acid (IBA) hormone
concentration on root and shoot development in stem cuttings of Warburgia ugandensis. Stem cuttings
were treated with three different levels (0.3, 0.6 and 0.8% w/w) of IBA concentration (including a controlno
IBA hormone) and propagated in three rooting media (milled pine bark, top forest soil and sand)
under non-misting propagators. Data on root and shoot development, callusing, number and length of
roots and shoots were analyzed using analysis of variance (ANOVA) and Chi square test. Callusing,
root and shoot development were significantly (p<0.05) influenced by rooting media and IBA
concentration. Milled pine bark and 0.8% w/w IBA concentration gave the highest percentages of stem
cuttings that callused, rooted and shooted (38, 37, 41% and 57, 41, 59%), respectively. Similarly, milled
pine bark and 0.8% w/w IBA concentration gave the greatest number and longest roots and shoots per
stem cutting. Vegetative propagation of W. ugandensis through stem cuttings can be appropriately
achieved by treating the cuttings with 0.8% w/w IBA hormone using milled pine bark as a growth medium.