Item Details

Title: Polymorphic microsatellite markers for the tsetse fly Glossina fuscipes fuscipes (Diptera: Glossinidae), a vector of human African trypanosomiasis

Date Published: 2008
Author/s: J . E . BROWN, K . J . KOMATSU, P. P. ABILA, A. S. ROBINSON, L. M. A. OKEDI, N. DYER, M. J. DONNELLY, M. A. SLOTMAN and A. CACCONE
Data publication:
Funding Agency : Yale Institute for Biospheric Studies, EANETT, L.M.A.O, Ugandan government and the Leverhulme Trust Tsetse network.
Copyright/patents/trade marks: The Authors, Blackwell Publishing Ltd
Journal Publisher: Molecular Ecology Resources
Affiliation: Department of Ecology and Evolutionary Biology, Yale University, 21 Sachem Street, New Haven, CT 06511, USA,
†National Livestock Resources Research Institute, PO Box 96, Tororo, Uganda, ‡Joint FAO/IAEA Programme of Nuclear Techniques
in Food and Agriculture, Insect Pest Control Sub-Programme, International Atomic Energy Agency, A-1400 Vienna, Austria,
§Vector Research Group, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK
Keywords: genetic marker, Glossina fuscipes, microsatellite loci, sleeping sickness, tsetse

Abstract:

Our understanding of Glossina fuscipes fuscipes, a major vector of sleeping sickness, has
been severely constrained by a lack of genetic markers for mapping and population genetic
studies. Here we present 10 newly developed microsatellite loci for this tsetse species.
Heterozygosity levels in Moyo, an Ugandan population, averaged 0.57, with only two loci
showing very low heterozygosity. Five loci carried more than six alleles. Together with five
recently published microsatellite loci, this brings the number of available microsatellite loci
for this species to 15. Their availability will greatly facilitate future studies on the genetics
of this important human disease vector.