Item Details

Title: Susceptibility of Ugandan Trypanosoma brucei rhodesienseisolated from man and animal reservoirs to diminazene,isometamidium and melarsoprol

Date Published: 1997
Author/s: Enoch Matovu, Monika Iten, John C. K. Enyaru, Cecile Schmid, George W. Lubega, Reto Brun and Ronald Kaminsky
Data publication:
Funding Agency : Swiss Development Cooperation (DEH/SDC)
Copyright/patents/trade marks: Blackwell Science Ltd
Journal Publisher: Tropical Medicine and International Health
Affiliation: Livestock Health Research Institute, Tororo, Uganda2Swiss Tropical Institute, Basel, Switzerland3Faculty of Veterinary Medicine, Makerere University, Kampala, Uganda
Keywords: Trypanosoma brucei spp., T. b. rhodesiense, animal reservoir, drug susceptibility, drug resistance


Thirty-six Trypanosoma brucei spp. stocks isolated from man and domestic animals insouth-east Uganda were studied for susceptibility to diminazene, isometamidium andmelarsoprol in vitro. All stocks were susceptible to melarsoprol. One T. b. rhodesiense stockisolated from a sleeping sickness patient showed reduced susceptibility to the veterinarydrugs diminazene and isometamidium. More than 100 ng/ml diminazene or 0.78 ng/mlisometamidium were required to eliminate that stock during 10 days drug exposure. Incontrast, the remaining stocks were eliminated by 0.8–6.3 ng/ml diminazene and0.01–0.20 ng/ml isometamidium. Clones derived from the resistant T. b. rhodesiense stockshowed reduced susceptibility to isometamidium and diminazene comparable to the parentalpopulation. Control of sleeping sickness by treatment of the animal reservoir could,therefore, face serious problems since drug-resistant stocks as reported here would mostlikely not be eliminated by recommended doses of diminazene and isometamidium.