Item Details

Title: Symptoms, aetiology and serological analysis of sweetpotato virus disease in Uganda

Date Published: 1998
Author/s: Gibson, R.W., Mpembe, I., Alicai, T., Carey, E. E., Mwanga, R.O.M., Seal, S. E. and Vettem, H.,
Data publication:
Funding Agency : British Overseas Development Administration ofthe United Kingdom, Holdback Project R5878 and theRNRRS-funded A0519
Copyright/patents/trade marks: BSPP
Journal Publisher: Plant Pathology,
Affiliation: NRI, University of Greenwich, Central Ave, Chatham Maritime, Kent ME4 4TB, UK;bNamulonge Agricultural and Animal ProductionResearch Institute, PO Box 7084, Kampala, Uganda;cCIP, Regional Of?ce for SubSaharan Africa, PO Box 25171, Nairobi, Kenya;dDepartment of Horticultural Science, Box 7609, North Carolina State University, Raleigh, NC 27695–7609, USA; andeInstitut fu¨r Biochemie und P?anzenvirologie, Biologische Bundesanstalt fu¨r Land und Forstwirtschaft, Messeweg 11/12,38104 Braunschweig, Germany
Keywords: aetiology, serological analysis, sweet potato virus disease, Uganda

Abstract:

Sweet potato virus disease (SPVD) is the name used to describe a range of severe symptoms in different cultivars ofsweet potato,comprisingoverallplantstunting combinedwith leaf narrowingand distortion,and chlorosis,mosaic orvein-clearing. Affected plants of various cultivars were collected from several regions of Uganda. All samplescontained the aphid-borne sweet potato feathery mottle potyvirus (SPFMV) and almost all contained the white?y-borne sweet potato chlorotic stunt closterovirus (SPCSV). SPCSV was detected by a mix of monoclonal antibodies(MAb)previouslyshowntoreact onlyto aKenyanisolate ofSPCSV,butnot byamixtureofMAb thatdetected SPCSVisolates from Nigeria and other countries. Sweet potato chlorotic ?eck virus (SPCFV) and sweet potato mild mottleipomovirus (SPMMV) were seldom detected in SPVD-affected plants, while sweet potato latent virus (SPLV) wasnever detected. Isolates of SPFMVand SPCSVobtained by insect transmissionstogether induced typical symptoms ofSPVD when graft-inoculated to virus-free sweet potato.SPCSValone caused stunting and either purpling or yellowingof middle and lower leaves when graft-inoculated to virus-free plants of two cultivars. Similarly diseased naturallyinoculated ?eld plants were shown consistently to contain SPCSV. Both this disease and SPVD spread rapidly in asweet potato crop.