Abstract:

The main aim of this study was to develop closely linked markers for rust resistance
landrace Aus27284 was also performed.
Close SNP-Ar45 associations were identified using the iSelect 90K. Infinium
array, five SNP markers co-segregated with Lr48 and IWB70I47 mapped 0.3 cM
proximal to this gene. Kompetitive allele-specific PCR (K.ASP) assays were developed
for linked SNPs. In contrast, the K.ASP markers developed from the iSelect 90K
Infinium SNP array for Lr49 did not result in close marker-trail association. Sequence
comparison of How sorted chromosome 4B from parents VL404 (Lr49) and WL711
(Jr49) resulted in dose association of sunKASP_21 (0.4 cM) with Lr49.
The Arina/Cezanne RIL population was used to develop markers linked with
Sr48 using the DArTseq platform and the iSelect 90K Infinium SNP array. DArTseq
based linkage map located Sr48 on the short arm of chromosome 2D. Marker siin590
derived from a DArTseq marker mapped 0.4 cM distal to Sr48. Sr48 was earlier mapped
repulsion linkage with Yrl. The detection of 2AL-2DS translocation in Fomo through
genomic in-situ hybridisation (GISH) appears to have resulted in pseudo-linkage to
locate Sr48 in chromosome 2AL.
Aus27284 was susceptible to stripe rust at the seedling stage and exhibited
resistance in field experiments. Genetic analysis showed monogenic segregation and
the resistance gene was temporarily designated YrAlVH. YrAWll was located on
xi
I
genes Lr48, Lr49 and Sr48. In addition, mapping of adult plant stripe rust resistance in a
in the long ami of chromosome 2A in the Arina/Fomo RIL population based on chromosome 3 BL near the centromere and KASP 65624/KASP 58449 and
KASP 53113 flanked this locus.
The closely linked markers identified in this study were tested on a set of
Australian and/or Nordic wheat genotypes to validate their suitability for markerassisted
selection (MAS). The results indicated that IWB70147, simKASP_21, sun590
and KASP 53113 can be used for MAS of Lr48, Lr49, Sr48 and YrA W11. respectively.